PRISM24™ & PACE24™ • Over Four Decades of Leadership in Urine Hormone Testing

Urinary Hormone & Metabolite Testing

Measure the hormones. Understand the pathways. See the bigger picture. Meridian Valley Laboratory pioneered commercial urine hormone testing for practitioner use more than four decades ago—capturing steroid hormone production and downstream metabolism across an entire 24-hour day rather than at a single moment in time.

Order PRISM24™ / PACE24™ Kits 24-Hr & Dried Urine Instructions

Why Test Hormones?

Parent Hormones + Downstream Metabolic Pathways

Hormone health is complex. Hormones function as an interconnected system, influencing metabolism, energy, mood, sleep, reproductive function, body composition, stress response, and many other aspects of health. Because these systems interact closely, symptoms of imbalance often overlap and can be difficult to evaluate from clinical presentation alone.

For practitioners evaluating hormone health or managing Bio-identical Hormone Replacement Therapy (BHRT), hormone levels are only part of the picture. How hormones are metabolized can be equally important. Measuring parent hormones together with their downstream metabolites provides a complete view of hormone production, metabolism, and the enzymatic pathways through which hormones are processed.

More Than 40 Years of Experience

PRISM24™ & PACE24™ 24-Hour Urine Assessments

Meridian Valley Laboratory pioneered commercial urine hormone testing for practitioner use in 1976. That experience continues today with advanced 24-hour liquid and dried urine hormone assessments:

• PRISM24™ Comprehensive Assessment: Provides an integrated view of up to 35+ primary hormones, metabolites, and enzymatic ratios across estrogens, androgens, progestins, mineralocorticoids, glucocorticoids, growth hormone, melatonin, and oxytocin.
• PACE24™ Targeted Assessment: Offers a focused 24-hour urinary steroid and adrenal evaluation ideally suited for targeted baseline assessment and ongoing BHRT monitoring.
• Testosterone Metabolites Profile: Measures 5-alpha DHT, protective 3-beta Adiol, and 3-alpha Adiol (including the ratio of 3β-Adiol to 3α-Adiol + DHT) for prostate, hair loss, and androgen pathway clarity.

Clinical Symptom Correlation

Symptoms of Hormonal Imbalance in Women & Men

Because endocrine pathways interact closely, evaluating all major steroid and peptide hormone families simultaneously helps practitioners pinpoint the true biochemical driver of patient symptoms.

Estrogens & Metabolites

Estrogen (E1, E2, E3 & 2/4/16-OH)

Low: Hot flashes, night sweats, vaginal dryness, brain fog, bone loss, joint pain, dry skin, low libido.
Excess / Dominance: Weight gain (hips/thighs), fibrocystic breasts, heavy menses, mood swings, water retention, migraines, gynecomastia in men.

Progestins & Pregnanediol

Progesterone

Low: Anxiety, insomnia, PMS, irregular or heavy menstrual cycles, luteal phase defect, breast tenderness, irritability.
Excess: Drowsiness, mild dizziness, bloating, or sluggish bowel motility during excessive supplementation.

Androgens & 5α/5β Pathways

Testosterone & DHEA

Low: Fatigue, loss of muscle mass and strength, decreased libido, erectile dysfunction, depressed mood,visceral fat gain, poor exercise recovery.
Excess / 5α-Reductase Preference: Acne, hirsutism, PCOS, scalp hair thinning, irritability, prostatic enlargement.

HPA Axis & Adrenal Reserve

Glucocorticoids (Cortisol & Cortisone)

Low (Adrenal Depletion): Morning fatigue, salt cravings, orthostatic dizziness, hypoglycemia, chronic inflammation, slow recovery from stress.
Excess: Central adiposity, "wired and tired" insomnia, elevated blood pressure, insulin resistance, bone demineralization, immune suppression.

Metabolic & Somatotropic

Thyroid & Growth Hormone

Low Thyroid / Low GH: Cold intolerance, weight gain, constipation, dry skin, thinning hair, reduced bone density, decreased lean muscle mass, thin skin, and impaired deep sleep recovery.

Circadian & Neuroendocrine

Melatonin (6-Sulfatoxymelatonin) & Oxytocin

Low Melatonin: Difficulty falling or staying asleep, circadian rhythm disruption, reduced nocturnal antioxidant defense.
Low Oxytocin: Reduced stress resilience, social disconnection, heightened pain sensitivity, and impaired emotional bonding.